Hotspot

accD-psaI

LSC · 61543-62132 · 590 bp

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Pi 0.0463

MarkerSeek score 64.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 41
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCATGCTTTCTTTCCTTTGAC	CCATTGCAATTGCCGGAAAT	40.9	45.0	57.1	58.0	695-858 bp	1.0	62.1
accD-psaI_p2	CCATGCTTTCTTTCCTTTGACT	CCATTGCAATTGCCGGAAAT	40.9	45.0	57.1	58.0	694-857 bp	1.0	62.0
accD-psaI_p3	TCCATGCTTTCTTTCCTTTGAC	AAGAAGCCATTGCAATTGCC	40.9	45.0	57.1	57.9	701-864 bp	1.0	61.4
accD-psaI_p4	CCATGCTTTCTTTCCTTTGACT	AAGAAGCCATTGCAATTGCC	40.9	45.0	57.1	57.9	700-863 bp	1.0	61.4
accD-psaI_p5	CTCCATGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	40.9	45.0	56.9	58.0	696-859 bp	1.0	59.9