Hotspot

atpH-atpI

LSC · 14521-15487 · 967 bp

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Pi 0.029

MarkerSeek score 51.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 42
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GTTGTCTCGCGATTCCTTCT	GGCAAATAGGGGGTTTCCAA	50.0	50.0	58.0	58.1	1756-1768 bp	1.0	82.0
atpH-atpI_p2	GTTGTCTCGCGATTCCTTCT	TTGGCAAATAGGGGGTTTCC	50.0	50.0	58.0	58.1	1758-1770 bp	1.0	82.0
atpH-atpI_p3	GAAGGAAGAAGGCGAGTCAG	GGCAAATAGGGGGTTTCCAA	55.0	50.0	58.0	58.1	2054-2066 bp	1.0	81.8
atpH-atpI_p4	GAAGGAAGAAGGCGAGTCAG	TTGGCAAATAGGGGGTTTCC	55.0	50.0	58.0	58.1	2056-2068 bp	1.0	81.8
atpH-atpI_p5	GTTGTCTCGCGATTCCTTCT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1124 bp	0.666667	66.1