Hotspot

trnS-GCU-trnG-UCC

LSC · 8286-8985 · 700 bp

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Pi 0.0248

MarkerSeek score 71.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 26
pi: 0.0248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAAGGACCCC	50.0	50.0	58.0	57.8	812-1003 bp	1.0	81.2
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAAGGACCCC	50.0	50.0	58.0	57.8	811-1002 bp	1.0	81.2
trnS-GCU-trnG-UCC_p3	TGGACGGAAAGAGAGGGATT	TGAATCAAACGAAGGACCCC	50.0	50.0	58.0	57.8	869-1060 bp	1.0	80.7
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GGGGCCAATAGAACGAATCA	50.0	50.0	58.0	57.7	781-972 bp	1.0	80.2
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAAGGACCCC	50.0	50.0	58.2	57.8	866-1057 bp	1.0	79.9