Hotspot

trnQ-UUG-psbK

LSC · 6817-7346 · 530 bp

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Pi 0.0302

MarkerSeek score 53.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 24
pi: 0.0302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATCATGCCTTTCAAGTCGCA	CTTCAGGCAATTTCGCGAAG	45.0	50.0	58.2	58.1	1325-1431 bp	1.0	84.1
trnQ-UUG-psbK_p2	GATCTTCCAAGTGACGCGAT	CTTCAGGCAATTTCGCGAAG	50.0	50.0	58.1	58.1	850-934 bp	1.0	82.3
trnQ-UUG-psbK_p3	TGGATATGTTCTGGGACGGA	CTTCAGGCAATTTCGCGAAG	50.0	50.0	57.8	58.1	697-777 bp	1.0	82.1
trnQ-UUG-psbK_p4	TCTTCCAAGTGACGCGATTT	CTTCAGGCAATTTCGCGAAG	45.0	50.0	57.8	58.1	848-932 bp	1.0	81.5
trnQ-UUG-psbK_p5	GATCTTCCAAGTGACGCGAT	TTACGTCCTGGGTCGTTAGA	50.0	50.0	58.1	57.8	1423-1523 bp	1.0	80.6