Hotspot

ycf2-trnH-GUG

IRa · 156211-156428 · 218 bp

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Pi 0.0615
MarkerSeek score 74.10
Primer available yes
Label ycf2-trnH-GUG

Feature Metadata

label_name
ycf2-trnH-GUG
feature_type
igs
region
IRa
length_bp
218
variable_sites
26
pi
0.0615
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnH-GUG_p1 ACTGAATTGAATTGGGTCCATG GTTGGGCGCTTTAACCATTC 40.9 50.0 56.9 58.0 353-355 bp 1.0 61.4
ycf2-trnH-GUG_p2 TCCTACTGAATTGAATTGGGTCC GTTGGGCGCTTTAACCATTC 43.5 50.0 58.1 58.0 357-359 bp 1.0 60.8
ycf2-trnH-GUG_p3 ACTGAATTGAATTGGGTCCATG ATTGGCATGCATCCAGTAGG 40.9 50.0 56.9 58.0 402-404 bp 1.0 60.7
ycf2-trnH-GUG_p4 TCCTACTGAATTGAATTGGGTCC ATTGGCATGCATCCAGTAGG 43.5 50.0 58.1 58.0 406-408 bp 1.0 60.1
ycf2-trnH-GUG_p5 ACTGAATTGAATTGGGTCCA ATTGGCATGCATCCAGTAGG 40.0 50.0 54.8 58.0 402-404 bp 1.0 59.8