Hotspot

ndhF-rpl32

SSC · 116956-117103 · 148 bp

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Pi 0.0626
MarkerSeek score 77.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
148
variable_sites
20
pi
0.0626
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TATCAAATCTGTTGGGGGCG CCAAATATTTTTACGAATACGCT 50.0 30.4 57.9 53.9 307-308 bp 1.0 45.5
ndhF-rpl32_p2 TATCAAATCTGTTGGGGGCG TCCAAATATTTTTACGAATACGCT 50.0 29.2 57.9 55.3 308-309 bp 1.0 45.4
ndhF-rpl32_p3 TATCAAATCTGTTGGGGGCG TCCAAATATTTTTACGAATACGCTT 50.0 28.0 57.9 56.0 308-309 bp 1.0 45.4
ndhF-rpl32_p4 TATCAAATCTGTTGGGGGCG TTCCAAATATTTTTACGAATACGCT 50.0 28.0 57.9 56.0 309-310 bp 1.0 45.4
ndhF-rpl32_p5 TATCAAATCTGTTGGGGGCG TCCAAATATTTTTACGAATACGC 50.0 30.4 57.9 53.9 308-309 bp 1.0 45.4