Hotspot

rrn5-trnR-ACG

IRb · 112557-112808 · 252 bp

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Pi 0.082

MarkerSeek score 87.80

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 252
variable_sites: 38
pi: 0.082
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	CCTTGTTCCTACATGACCCG	CGTCGAAGCGAAAATAACCG	55.0	50.0	58.0	57.9	1084 bp	0.2	47.4
rrn5-trnR-ACG_p2	CCTTGTTCCTACATGACCCG	ACACCGTCGAAGCGAAAATA	55.0	45.0	58.0	57.9	1088 bp	0.2	47.3
rrn5-trnR-ACG_p3	CCTCGCAATCACTAGCCAAT	CGTCGAAGCGAAAATAACCG	50.0	50.0	58.0	57.9	917 bp	0.2	47.2
rrn5-trnR-ACG_p4	TGTCCCCTCCAGTCAAGAAT	CGTCGAAGCGAAAATAACCG	50.0	50.0	58.0	57.9	942 bp	0.2	47.2
rrn5-trnR-ACG_p5	GTCAAGTGGAAGTGCAGTGA	CGTCGAAGCGAAAATAACCG	50.0	50.0	58.0	57.9	1145 bp	0.2	47.2