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rps12-clpP

LSC · 75057-75425 · 369 bp

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Pi 0.0528

MarkerSeek score 85.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 43
pi: 0.0528
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GTACATGTTCCTCGACGCTG	GAAGACTATGCCTTCGCCAT	55.0	50.0	58.7	57.8	501-578 bp	1.0	82.7
rps12-clpP_p2	GTACATGTTCCTCGACGCTG	AAGAAGACTATGCCTTCGCC	55.0	50.0	58.7	57.7	503-580 bp	1.0	82.1
rps12-clpP_p3	AGTACATGTTCCTCGACGCT	GAAGACTATGCCTTCGCCAT	50.0	50.0	58.8	57.8	502-579 bp	1.0	81.9
rps12-clpP_p4	TACATGTTCCTCGACGCTGA	GAAGACTATGCCTTCGCCAT	50.0	50.0	58.8	57.8	500-577 bp	1.0	81.9
rps12-clpP_p5	TAGTACATGTTCCTCGACGC	GAAGACTATGCCTTCGCCAT	50.0	50.0	57.2	57.8	503-580 bp	1.0	81.8