Hotspot

accD-psaI

LSC · 62900-63482 · 583 bp

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Pi 0.0362

MarkerSeek score 73.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 40
pi: 0.0362
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGACAGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	881-906 bp	1.0	87.3
accD-psaI_p2	CTATGGGATCCGTAGTGGGT	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1101-1126 bp	1.0	86.8
accD-psaI_p3	ACCCGATTGATTGAGTACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1072-1097 bp	1.0	86.4
accD-psaI_p4	GGTGACAGCCAGTTTTGGTA	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1400-1424 bp	1.0	86.1
accD-psaI_p5	CTATGGGATCCGTAGTGGGT	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	1620-1644 bp	1.0	85.4