Hotspot

rbcL-accD

LSC · 59253-62272 · 3020 bp

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Pi 0.0407

MarkerSeek score 73.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 3020
variable_sites: 213
pi: 0.0407
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CATTCGGAGTTGCGATGAGA	CTTAGTCCACACCTGTGCTC	50.0	55.0	58.1	57.9	559-675 bp	1.0	90.7
rbcL-accD_p2	ACGACGGTTGTCCTATTTGG	TCTCATCGCAACTCCGAATG	50.0	50.0	57.9	58.1	853-969 bp	1.0	90.7
rbcL-accD_p3	CATTCGGAGTTGCGATGAGA	CCAAATAGGACAACCGTCGT	50.0	50.0	58.1	57.9	767-883 bp	1.0	90.7
rbcL-accD_p4	ACGACGGTTGTCCTATTTGG	CTTAGTCCACACCTGTGCTC	50.0	55.0	57.9	57.9	602-718 bp	1.0	90.6
rbcL-accD_p5	ATTCGGAGTTGCGATGAGAG	CTTAGTCCACACCTGTGCTC	50.0	55.0	57.8	57.9	558-674 bp	1.0	89.6