Hotspot

psbZ-trnG-UCC

LSC · 38396-38712 · 317 bp

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Pi 0.053

MarkerSeek score 74.00

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 317
variable_sites: 29
pi: 0.053
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CAACCACTCAGCCATCTCTC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.0	58.0	1163-1171 bp	1.0	82.7
psbZ-trnG-UCC_p2	CAACCACTCAGCCATCTCTC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	1465-1473 bp	1.0	82.3
psbZ-trnG-UCC_p3	ATCCCACATGCCCATTTGTT	CGTTGTTTTTCGACCGGAAG	45.0	50.0	58.0	58.0	1317-1325 bp	1.0	82.3
psbZ-trnG-UCC_p4	TCGTTATGATACCGTCCGGA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1597-1605 bp	1.0	82.0
psbZ-trnG-UCC_p5	CGTTATGATACCGTCCGGAC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	1596-1604 bp	1.0	81.9