Hotspot

trnC-GCA-petN

LSC · 29554-30423 · 870 bp

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Pi 0.0566

MarkerSeek score 91.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 93
pi: 0.0566
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 91.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	855-935 bp	1.0	90.1
trnC-GCA-petN_p2	GTATCAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	907-987 bp	1.0	89.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	873-953 bp	1.0	87.9
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTAGAGCCCACTTCTTCCCC	50.0	55.0	58.1	58.7	918-998 bp	1.0	85.3
trnC-GCA-petN_p5	GTATCAATTTGGCGGCATGG	TTAGAGCCCACTTCTTCCCC	50.0	55.0	57.9	58.7	970-1050 bp	1.0	84.4