Hotspot

trnH-GUG-psbA

LSC · 250-606 · 357 bp

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Pi 0.0536
MarkerSeek score 78.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
357
variable_sites
37
pi
0.0536
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GGTTTGTCTTGTGTCTCCGT CTGACGGTATGCCTCTAGGA 50.0 55.0 58.1 58.0 843-1031 bp 0.6 69.0
trnH-GUG-psbA_p2 GGTTTGTCTTGTGTCTCCGT GAGCTTAGTTTCCGTCTGGG 50.0 55.0 58.1 58.0 955-1143 bp 0.6 68.9
trnH-GUG-psbA_p3 GGTTTGTCTTGTGTCTCCGT GAGTGGGAGCTTAGTTTCCG 50.0 55.0 58.1 58.0 961-1149 bp 0.6 68.9
trnH-GUG-psbA_p4 GGTTTGTCTTGTGTCTCCGT GGAGCTTAGTTTCCGTCTGG 50.0 55.0 58.1 58.0 956-1144 bp 0.6 68.9
trnH-GUG-psbA_p5 GGTTTGTCTTGTGTCTCCGT GGGAGCTTAGTTTCCGTCTG 50.0 55.0 58.1 58.0 957-1145 bp 0.6 68.9