Hotspot

ycf3

LSC · 43708-45673 · 1966 bp

Back to Deutzia

Pi 0.00174

MarkerSeek score 36.20

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1966
variable_sites: 14
pi: 0.00174
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ATGGGACCAACAAATAAGAT	TCATTTTCCCGCGAGAGAAG	35.0	50.0	52.0	58.0	2094-2106 bp	1.0	41.4
ycf3_p2	ATGGGACCAACAAATAAGAT	CCCGCGAGAGAAGTAATGAG	35.0	55.0	52.0	57.9	2087-2099 bp	1.0	41.4
ycf3_p3	ATGGGACCAACAAATAAGAT	GATCATTTTCCCGCGAGAGA	35.0	50.0	52.0	57.8	2096-2108 bp	1.0	41.4
ycf3_p4	ATGGGACCAACAAATAAGAT	TCCCGCGAGAGAAGTAATGA	35.0	50.0	52.0	58.2	2088-2100 bp	1.0	41.4
ycf3_p5	ATGGGACCAACAAATAAGAT	CATTTTCCCGCGAGAGAAGT	35.0	50.0	52.0	58.3	2093-2105 bp	1.0	41.4