Hotspot

ycf1

SSC · 126338-132007 · 5670 bp

Back to Deutzia

Pi 0.00308

MarkerSeek score 41.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 74
pi: 0.00308
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTTTCGTGACTTGTTTCA	AGAACTGTTCGATTAATCCGT	35.0	38.1	53.3	55.1	181 bp	1.0	43.1
ycf1_p2	TCAATTCTTTCGTGACTTGTTTCA	AGAACTGTTCGATTAATCCGT	33.3	38.1	57.6	55.1	185 bp	1.0	43.0
ycf1_p3	TCAATTCTTTCGTGACTTGTTTCA	GAAGAACTGTTCGATTAATCCGT	33.3	39.1	57.6	57.0	187 bp	1.0	43.0
ycf1_p4	TCAATTCTTTCGTGACTTGTTTCA	AAGAACTGTTCGATTAATCCGT	33.3	36.4	57.6	55.8	186 bp	1.0	43.0
ycf1_p5	CAATTCTTTCGTGACTTGTTTCA	AGAACTGTTCGATTAATCCGT	34.8	38.1	56.2	55.1	184 bp	1.0	43.0