Hotspot

rpoC2

LSC · 17153-21316 · 4164 bp

Back to Deutzia

Pi 0.004

MarkerSeek score 49.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4164
variable_sites: 72
pi: 0.004
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CTCCAGTGCATTTCTCCCTC	TTGGTTCATCCGACACGTAC	55.0	50.0	58.0	57.9	257 bp	1.0	81.3
rpoC2_p2	CTCCAGTGCATTTCTCCCTC	ATTTGGTTCATCCGACACGT	55.0	45.0	58.0	57.8	259 bp	1.0	80.8
rpoC2_p3	CTCCAGTGCATTTCTCCCTC	CATGGACATCCGGCTTTTCT	55.0	50.0	58.0	58.2	233 bp	1.0	80.2
rpoC2_p4	CTCCAGTGCATTTCTCCCTC	TCATGGACATCCGGCTTTTC	55.0	50.0	58.0	58.2	234 bp	1.0	80.1
rpoC2_p5	GCGCATGATACACATCGGTA	TTGGTTCATCCGACACGTAC	50.0	50.0	58.2	57.9	277 bp	1.0	79.7