Hotspot

matK

LSC · 1999-3516 · 1518 bp

Back to Deutzia

Pi 0.00275

MarkerSeek score 54.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 19
pi: 0.00275
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCGTTCCGATTAGTTAGGGA	ACCCCCAAAATCCTCTACCT	45.0	50.0	55.4	57.6	1603-1612 bp	1.0	57.2
matK_p2	ATCGTTCCGATTAGTTAGGGA	ACCCCCAAAATCCTCTACCT	42.9	50.0	56.0	57.6	1604-1613 bp	1.0	53.8
matK_p3	TCGTTCCGATTAGTTAGGGAT	ACCCCCAAAATCCTCTACCT	42.9	50.0	56.0	57.6	1603-1612 bp	1.0	53.8
matK_p4	TCGTTCCGATTAGTTAGGGA	AACCCCCAAAATCCTCTACC	45.0	50.0	55.4	56.8	1604-1613 bp	1.0	50.5
matK_p5	ATCGTTCCGATTAGTTAGGGAT	ACCCCCAAAATCCTCTACCT	40.9	50.0	56.5	57.6	1604-1613 bp	1.0	50.1