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ccsA-ndhD

SSC · 117862-118112 · 251 bp

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Pi 0.0104

MarkerSeek score 54.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 9
pi: 0.0104
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCAATTGTGGCTTCT	ACCCGGATTTCGTTCTTTCA	40.0	45.0	55.9	57.4	436-442 bp	1.0	62.2
ccsA-ndhD_p2	AATTCTGCAATTGTGGCTTCT	ACCCGGATTTCGTTCTTTCA	38.1	45.0	56.7	57.4	437-443 bp	1.0	60.5
ccsA-ndhD_p3	ATTCTGCAATTGTGGCTTCT	CCCGGATTTCGTTCTTTCAC	40.0	50.0	55.9	57.1	435-441 bp	1.0	59.5
ccsA-ndhD_p4	CAAATTCTGCAATTGTGGCT	ACCCGGATTTCGTTCTTTCA	40.0	45.0	55.4	57.4	439-445 bp	1.0	58.3
ccsA-ndhD_p5	AAATTCTGCAATTGTGGCTTCT	ACCCGGATTTCGTTCTTTCA	36.4	45.0	57.4	57.4	438-444 bp	1.0	58.1