Hotspot

rpl32-trnL-UAG

SSC · 115796-116717 · 922 bp

Back to Deutzia

Pi 0.00838

MarkerSeek score 56.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 922
variable_sites: 28
pi: 0.00838
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCAAATTAGGCCCGCTCTTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1490-1661 bp	1.0	84.2
rpl32-trnL-UAG_p2	TCAAATTAGGCCCGCTCTTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1491-1662 bp	1.0	84.1
rpl32-trnL-UAG_p3	TCAAATTAGGCCCGCTCTTG	GCTGCATAGCCCAATACCAT	50.0	50.0	58.2	58.1	2086-2257 bp	1.0	83.3
rpl32-trnL-UAG_p4	TCAAATTAGGCCCGCTCTTG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1500-1671 bp	1.0	83.2
rpl32-trnL-UAG_p5	TCAAATTAGGCCCGCTCTTG	TCGAACCGAGATGCTTTAGC	50.0	50.0	58.2	58.1	1666 bp	0.090909	41.0