Hotspot

ycf4-cemA

LSC · 62330-63180 · 851 bp

Back to Deutzia

Pi 0.00581

MarkerSeek score 61.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 21
pi: 0.00581
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	962-986 bp	1.0	80.8
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	976-1000 bp	1.0	80.1
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	974-998 bp	1.0	79.7
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AGAAATCCACCAGGGCAAAA	45.0	45.0	58.1	57.6	961-985 bp	1.0	78.2
ycf4-cemA_p5	AGCTGCTGAATTGGCCTATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.6	58.1	972-996 bp	1.0	77.9