Hotspot

trnF-GAA-ndhJ

LSC · 49744-50414 · 671 bp

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Pi 0.00537

MarkerSeek score 56.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 16
pi: 0.00537
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	810-842 bp	1.0	81.1
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	845-877 bp	1.0	80.2
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	798-830 bp	1.0	80.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	774-779 bp	0.909091	77.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	809-814 bp	0.909091	76.3