Hotspot

trnL-UAA-trnF-GAA

LSC · 49298-49670 · 373 bp

Back to Deutzia

Pi 0.00712

MarkerSeek score 42.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 10
pi: 0.00712
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	426-429 bp	1.0	78.6
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	438-441 bp	1.0	77.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CCATGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	479-482 bp	1.0	77.2
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TAATCATGTGCCATGCCAGG	50.0	50.0	57.5	58.3	489-492 bp	1.0	75.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	462-465 bp	0.909091	74.4