Hotspot

rpoB-trnC-GCA

LSC · 27553-28763 · 1211 bp

Back to Deutzia

Pi 0.00387

MarkerSeek score 56.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1211
variable_sites: 17
pi: 0.00387
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	TGAAAGGATTTGCAGTCCCC	50.0	50.0	58.0	58.1	1342-1386 bp	1.0	80.9
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	CGGATTTGAACTGGGGTGAA	50.0	50.0	58.0	58.1	1358-1402 bp	1.0	80.7
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATCAATGA	ACTGGGGTGAAAGGATTTGC	50.0	50.0	58.0	58.4	1349-1393 bp	1.0	78.5
rpoB-trnC-GCA_p4	CGTCAAGCCCTGATCAATGA	GGGGTGAAAGGATTTGCAGT	50.0	50.0	58.0	58.4	1346-1390 bp	1.0	78.5
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATCAATGA	TGGGGTGAAAGGATTTGCAG	50.0	50.0	58.0	58.4	1347-1391 bp	1.0	78.5