Hotspot

atpH-atpI

LSC · 14360-15263 · 904 bp

Back to Deutzia

Pi 0.00396

MarkerSeek score 55.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 15
pi: 0.00396
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.0	58.0	1004-1027 bp	1.0	81.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.9	58.0	1060-1083 bp	1.0	80.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.2	58.0	1005-1028 bp	1.0	80.2
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CAAGCGGTATCCAAGCTCTT	55.0	50.0	58.2	58.0	1064-1087 bp	1.0	80.0
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.8	58.0	1019-1042 bp	1.0	79.9