Hotspot

trnG-UCC-trnR-UCU

LSC · 10584-10701 · 118 bp

Back to Deutzia

Pi 0.031

MarkerSeek score 57.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 9
pi: 0.031
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	197-207 bp	1.0	67.8
trnG-UCC-trnR-UCU_p2	GCTAACGATGTGGGTTCGAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.1	56.9	187-197 bp	1.0	67.7
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	200-210 bp	1.0	67.5
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	203-213 bp	1.0	67.0
trnG-UCC-trnR-UCU_p5	TTCCAAGCTAACGATGTGGG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.9	56.9	194 bp	0.090909	26.4