Hotspot

trnK-UUU-rps16

LSC · 4279-5098 · 820 bp

Back to Deutzia

Pi 0.00658
MarkerSeek score 63.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
820
variable_sites
21
pi
0.00658
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTCGCTTCATCAA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 997-1029 bp 1.0 82.1
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1010-1042 bp 1.0 81.9
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1009-1041 bp 1.0 81.9
trnK-UUU-rps16_p4 CGAATCCCTCGCTTCATCAA AGAAGGCGGAGGTTTTCAAG 50.0 50.0 58.1 58.1 955-987 bp 1.0 81.6
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AGAAGGCGGAGGTTTTCAAG 55.0 50.0 57.9 58.1 967-999 bp 1.0 81.4