Hotspot

trnH-GUG-psbA

LSC · 77-428 · 352 bp

Back to Deutzia

Pi 0.0105

MarkerSeek score 51.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 18
pi: 0.0105
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGATCTAGCGGC	55.0	55.0	58.1	58.1	414-442 bp	1.0	85.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGATCTAGCGGC	50.0	55.0	57.9	58.1	428-456 bp	1.0	85.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGATCTAGCGGC	50.0	55.0	57.9	58.1	427-455 bp	1.0	85.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGATCTAGCGGC	50.0	55.0	57.9	58.1	429-457 bp	1.0	85.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	463-491 bp	1.0	83.7