Hotspot

psbA-ccs1

Genome · 116775-116867 · 93 bp

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Pi 0.108

MarkerSeek score 81.70

Primer available yes

Label psbA-ccs1

Feature Metadata

label_name: psbA-ccs1
feature_type: igs
region: Genome
length_bp: 93
variable_sites: 10
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-ccs1_p1	CCATAATGAAACGCCTTCGC	GGGAAATACAACAAGGGCGA	50.0	50.0	57.9	58.2	196 bp	1.0	81.9
psbA-ccs1_p2	CGCGTCTTTCTAAAGTTGCG	GGGAAATACAACAAGGGCGA	50.0	50.0	58.1	58.2	179 bp	1.0	81.9
psbA-ccs1_p3	CCATAATGAAACGCCTTCGC	ACAACAAGGGCGACTTTTGA	50.0	45.0	57.9	58.2	189 bp	1.0	81.5
psbA-ccs1_p4	CGCGTCTTTCTAAAGTTGCG	ACAACAAGGGCGACTTTTGA	50.0	45.0	58.1	58.2	172 bp	1.0	81.5
psbA-ccs1_p5	ACAACCAAACCAACCGATGT	GGGAAATACAACAAGGGCGA	45.0	50.0	58.2	58.2	259 bp	1.0	79.9