Hotspot

ycf35-psbA

Genome · 115216-115691 · 476 bp

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Pi 0.334

MarkerSeek score 84.70

Primer available yes

Label ycf35-psbA

Feature Metadata

label_name: ycf35-psbA
feature_type: igs
region: Genome
length_bp: 476
variable_sites: 145
pi: 0.334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35-psbA_p1	TGGAATCTCTAACTTATTTAATGCA	GCTCCAGCTGTAAATGCTTA	28.0	45.0	54.6	55.8	573-578 bp	1.0	52.0
ycf35-psbA_p2	TGGAATCTCTAACTTATTTAATGCA	GCTCCAGCTGTAAATGCTTAA	28.0	42.9	54.6	56.6	573-578 bp	1.0	52.0
ycf35-psbA_p3	ATGGAATCTCTAACTTATTTAATGCA	GCTCCAGCTGTAAATGCTTA	26.9	45.0	55.1	55.8	574-579 bp	1.0	52.0
ycf35-psbA_p4	TGGAATCTCTAACTTATTTAATGCAT	GCTCCAGCTGTAAATGCTTA	26.9	45.0	55.1	55.8	573-578 bp	1.0	52.0
ycf35-psbA_p5	CTTGTTTTCAAATTTGTAAAATGTGAC	GCTCCAGCTGTAAATGCTTA	25.9	45.0	55.9	55.8	521-526 bp	1.0	52.0