Hotspot

rps6-ccsA

Genome · 112477-113412 · 936 bp

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Pi 0.163

MarkerSeek score 74.50

Primer available yes

Label rps6-ccsA

Feature Metadata

label_name: rps6-ccsA
feature_type: igs
region: Genome
length_bp: 936
variable_sites: 82
pi: 0.163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps6-ccsA_p1	GTTAGAAGAATCATCATTTCGT	AGCAAAGAGTAAAATGCCAA	31.8	35.0	52.4	53.7	609-1040 bp	1.0	52.0
rps6-ccsA_p2	GTTAGAAGAATCATCATTTCGT	GCAAAGAGTAAAATGCCAAAAACA	31.8	33.3	52.4	57.6	608-1039 bp	1.0	52.0
rps6-ccsA_p3	GTTAGAAGAATCATCATTTCGT	AGCAAAGAGTAAAATGCCAAA	31.8	33.3	52.4	54.5	609-1040 bp	1.0	52.0
rps6-ccsA_p4	GTTAGAAGAATCATCATTTCGT	AGCAAAGAGTAAAATGCCAAAAAC	31.8	33.3	52.4	57.4	609-1040 bp	1.0	52.0
rps6-ccsA_p5	GTTAGAAGAATCATCATTTCGT	TCATAGCAAAGAGTAAAATGCCA	31.8	34.8	52.4	56.5	613-1044 bp	1.0	51.9