Hotspot

ccs1-psbA

Genome · 81920-82012 · 93 bp

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Pi 0.108

MarkerSeek score 81.70

Primer available yes

Label ccs1-psbA

Feature Metadata

label_name: ccs1-psbA
feature_type: igs
region: Genome
length_bp: 93
variable_sites: 10
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1-psbA_p1	GGGAAATACAACAAGGGCGA	CCATAATGAAACGCCTTCGC	50.0	50.0	58.2	57.9	196 bp	1.0	81.9
ccs1-psbA_p2	GGGAAATACAACAAGGGCGA	CGCGTCTTTCTAAAGTTGCG	50.0	50.0	58.2	58.1	179 bp	1.0	81.9
ccs1-psbA_p3	ACAACAAGGGCGACTTTTGA	CCATAATGAAACGCCTTCGC	45.0	50.0	58.2	57.9	189 bp	1.0	81.5
ccs1-psbA_p4	ACAACAAGGGCGACTTTTGA	CGCGTCTTTCTAAAGTTGCG	45.0	50.0	58.2	58.1	172 bp	1.0	81.5
ccs1-psbA_p5	GGGAAATACAACAAGGGCGA	ACAACCAAACCAACCGATGT	50.0	45.0	58.2	58.2	259 bp	1.0	79.9