Hotspot

trnA-UGC-rnl

Genome · 70066-70125 · 60 bp

Back to Detonula

Pi 0.0667
MarkerSeek score 83.30
Primer available yes
Label trnA-UGC-rnl

Feature Metadata

label_name
trnA-UGC-rnl
feature_type
igs
region
Genome
length_bp
60
variable_sites
4
pi
0.0667
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-rnl_p1 CCCTGATAAGGGTGAGGTCT CTGAATGCCAAGGTATCCCC 55.0 55.0 57.8 58.0 224 bp 1.0 79.9
trnA-UGC-rnl_p2 CCCTGATAAGGGTGAGGTCT CGTCCTTCATCGCTTCTGAA 55.0 50.0 57.8 58.0 239 bp 1.0 79.8
trnA-UGC-rnl_p3 CCCTGATAAGGGTGAGGTCT TGCCAAGGTATCCCCCATAA 55.0 50.0 57.8 58.1 219 bp 1.0 79.3
trnA-UGC-rnl_p4 CCCTGATAAGGGTGAGGTCT GAGACCTCCGGATCATAGCT 55.0 55.0 57.8 58.1 295 bp 1.0 79.0
trnA-UGC-rnl_p5 CCCTGATAAGGGTGAGGTCT TCATCGCTTCTGAATGCCAA 55.0 45.0 57.8 57.9 233 bp 1.0 79.0