Hotspot

trnY-GUA-petG

Genome · 51556-51913 · 358 bp

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Pi 0.146

MarkerSeek score 78.60

Primer available yes

Label trnY-GUA-petG

Feature Metadata

label_name: trnY-GUA-petG
feature_type: igs
region: Genome
length_bp: 358
variable_sites: 50
pi: 0.146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-petG_p1	GTAAATCCGCTGCGTTATGC	AGTGGCACAAGAATCGAAGG	50.0	50.0	58.0	58.2	1019 bp	0.5	58.4
trnY-GUA-petG_p2	AAATCCGCTGCGTTATGCTA	AGTGGCACAAGAATCGAAGG	45.0	50.0	58.1	58.2	1017 bp	0.5	58.1
trnY-GUA-petG_p3	TAAATCCGCTGCGTTATGCT	AGTGGCACAAGAATCGAAGG	45.0	50.0	58.1	58.2	1018 bp	0.5	58.1
trnY-GUA-petG_p4	AAGGGGGCGGATTGTAAATC	AGTGGCACAAGAATCGAAGG	50.0	50.0	57.9	58.2	1032 bp	0.5	58.0
trnY-GUA-petG_p5	GTAAATCCGCTGCGTTATGC	GGCAGCTGTTGGATTCAGAT	50.0	50.0	58.0	58.2	680 bp	0.5	57.9