Hotspot

ycf45-ffs

Genome · 23100-23598 · 499 bp

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Pi 0.16

MarkerSeek score 79.20

Primer available yes

Label ycf45-ffs

Feature Metadata

label_name: ycf45-ffs
feature_type: igs
region: Genome
length_bp: 499
variable_sites: 78
pi: 0.16
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45-ffs_p1	AGCTTGCGATCGAAGTCAAT	TCCACTGCTCTACCAACTGA	45.0	50.0	58.0	58.0	1478-1488 bp	1.0	88.6
ycf45-ffs_p2	TTTCAGCTTGCGATCGAAGT	TCCACTGCTCTACCAACTGA	45.0	50.0	58.2	58.0	1482-1492 bp	1.0	87.1
ycf45-ffs_p3	AGCTTGCGATCGAAGTCAAT	AACTGAGCTATCCGGGCTTA	45.0	50.0	58.0	58.2	1464-1474 bp	1.0	87.0
ycf45-ffs_p4	AGCCAAAAACCCTTGGTTCA	TCCACTGCTCTACCAACTGA	45.0	50.0	58.1	58.0	1244 bp	0.5	58.9
ycf45-ffs_p5	CTGCATTTCAGCTTGCGATC	TCCACTGCTCTACCAACTGA	50.0	50.0	58.2	58.0	1487 bp	0.5	58.5