Hotspot

psbX-rbcS

Genome · 7729-8249 · 521 bp

Back to Detonula

Pi 0.142

MarkerSeek score 84.60

Primer available yes

Label psbX-rbcS

Feature Metadata

label_name: psbX-rbcS
feature_type: igs
region: Genome
length_bp: 521
variable_sites: 68
pi: 0.142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbX-rbcS_p1	CCTGCTGTTAAACTCGCGAT	CCCTGAAGGTAGCCGTTACT	50.0	55.0	58.6	58.8	544-587 bp	1.0	80.4
psbX-rbcS_p2	CCTGCTGTTAAACTCGCGAT	CAGTGTTCAAGCTAACCCTGA	50.0	47.6	58.6	57.9	559-602 bp	1.0	77.9
psbX-rbcS_p3	CCTGCTGTTAAACTCGCGAT	TGTTCAAGCTAACCCTGAAGG	50.0	47.6	58.6	57.9	556-599 bp	1.0	77.8
psbX-rbcS_p4	CCTGCTGTTAAACTCGCGAT	ACAGTGTTCAAGCTAACCCTG	50.0	47.6	58.6	58.1	560-603 bp	1.0	77.7
psbX-rbcS_p5	CCTGCTGTTAAACTCGCGAT	ACAGTGTTCAAGCTAACCCT	50.0	45.0	58.6	56.7	560-603 bp	1.0	76.3