Hotspot

psbA

Genome · 45309-48885 · 3577 bp

Back to Desmodesmus

Pi 0.205

MarkerSeek score 79.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 3577
variable_sites: 698
pi: 0.205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ATGTCAAGGCTGTTCTTTGC	TGATTGGTTTGAACGCAAAA	45.0	35.0	56.9	54.9	176 bp	0.5	26.5
psbA_p2	ATGTCAAGGCTGTTCTTTGC	TTGATTGGTTTGAACGCAAA	45.0	35.0	56.9	54.9	177 bp	0.5	26.5
psbA_p3	ATGTCAAGGCTGTTCTTTGC	TTTGATTGGTTTGAACGCAA	45.0	35.0	56.9	54.9	178 bp	0.5	26.5
psbA_p4	ATGTCAAGGCTGTTCTTTGC	TTTTGATTGGTTTGAACGCA	45.0	35.0	56.9	54.9	179 bp	0.5	26.5
psbA_p5	ATGTCAAGGCTGTTCTTTGC	TGATTGGTTTGAACGCAAAAA	45.0	33.3	56.9	55.7	176 bp	0.5	25.0