Hotspot

ccsA-psaB

Genome · 114047-114830 · 784 bp

Back to Desmodesmus

Pi 0.0256

MarkerSeek score 76.60

Primer available yes

Label ccsA-psaB

Feature Metadata

label_name: ccsA-psaB
feature_type: igs
region: Genome
length_bp: 784
variable_sites: 19
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaB_p1	ACATGAAAAGCAATTTGAACCCA	TGTTCAAGCTCGTTTAGTAGGT	34.8	40.9	58.0	57.4	948-982 bp	1.0	45.0
ccsA-psaB_p2	ACATGAAAAGCAATTTGAACCCA	AGCTCGTTTAGTAGGTTTAGCA	34.8	40.9	58.0	57.2	942-976 bp	1.0	45.0
ccsA-psaB_p3	ACATGAAAAGCAATTTGAACCCA	GCTCGTTTAGTAGGTTTAGCAC	34.8	45.5	58.0	57.2	941-975 bp	1.0	45.0
ccsA-psaB_p4	ACATGAAAAGCAATTTGAACCCA	ACCTGTTGCGTTATCAATTGT	34.8	38.1	58.0	56.4	966-1000 bp	1.0	44.9
ccsA-psaB_p5	ACATGAAAAGCAATTTGAACCCA	TGCGTTATCAATTGTTCAAGCT	34.8	36.4	58.0	57.2	960-994 bp	1.0	44.9