Hotspot

ODF51_pgt015-rps4

Genome · 93513-93818 · 306 bp

Back to Desmodesmus

Pi 0.0522
MarkerSeek score 79.60
Primer available yes
Label ODF51_pgt015-rps4

Feature Metadata

label_name
ODF51_pgt015-rps4
feature_type
igs
region
Genome
length_bp
306
variable_sites
14
pi
0.0522
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ODF51_pgt015-rps4_p1 GGAGAAAACGGGGATTCGAA GCACCTTTTCCTCGAAATGC 50.0 50.0 57.9 58.0 409-447 bp 1.0 85.6
ODF51_pgt015-rps4_p2 TTTGAATGTTTCTGCCCCCA GCACCTTTTCCTCGAAATGC 45.0 50.0 57.9 58.0 461-499 bp 1.0 85.4
ODF51_pgt015-rps4_p3 TCCCTTTCACGGAGAAAACG GCACCTTTTCCTCGAAATGC 50.0 50.0 58.1 58.0 419-457 bp 1.0 85.4
ODF51_pgt015-rps4_p4 TGAATGTTTCTGCCCCCATC GCACCTTTTCCTCGAAATGC 50.0 50.0 58.1 58.0 459-497 bp 1.0 85.0
ODF51_pgt015-rps4_p5 TTGAATGTTTCTGCCCCCAT GCACCTTTTCCTCGAAATGC 45.0 50.0 57.7 58.0 460-498 bp 1.0 83.6