Hotspot

infA-psbB

Genome · 66720-67141 · 422 bp

Back to Desmodesmus

Pi 0.0357

MarkerSeek score 77.30

Primer available yes

Label infA-psbB

Feature Metadata

label_name: infA-psbB
feature_type: igs
region: Genome
length_bp: 422
variable_sites: 13
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-psbB_p1	TGGTGACAAAGTGACTGTTGA	AAGTGTCATTGAACCTGCCC	42.9	50.0	57.7	58.4	557-612 bp	1.0	72.0
infA-psbB_p2	TGGTGACAAAGTGACTGTTGA	TGCACAGAAATTAGTCGCCC	42.9	50.0	57.7	58.6	508-563 bp	1.0	70.8
infA-psbB_p3	TGGTGACAAAGTGACTGTTGA	GCCCCGGGTCATTAATAACT	42.9	50.0	57.7	57.3	492-547 bp	1.0	70.1
infA-psbB_p4	TGGTGACAAAGTGACTGTTGA	CCCAACCAGCAACTAAAGCA	42.9	50.0	57.7	58.7	540-595 bp	1.0	69.7
infA-psbB_p5	TGGTGACAAAGTGACTGTTGA	TGCCCAACCAGCAACTAAAG	42.9	50.0	57.7	58.7	542-597 bp	1.0	69.7