Hotspot

chlB-ODF51_pgt007

Genome · 60104-61955 · 1852 bp

Back to Desmodesmus

Pi 0.0968

MarkerSeek score 82.70

Primer available yes

Label chlB-ODF51_pgt007

Feature Metadata

label_name: chlB-ODF51_pgt007
feature_type: igs
region: Genome
length_bp: 1852
variable_sites: 175
pi: 0.0968
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-ODF51_pgt007_p1	CTGGACCAGCATACATCCAA	CAGGATTTGAACCTGCGACT	50.0	50.0	57.3	58.2	1951-2067 bp	1.0	81.5
chlB-ODF51_pgt007_p2	CTGGACCAGCATACATCCAA	ATTATGAGCCCCACGAGCTA	50.0	50.0	57.3	58.3	1926-2042 bp	1.0	80.8
chlB-ODF51_pgt007_p3	CTGGACCAGCATACATCCAA	GAACCTGCGACTTTGGGATT	50.0	50.0	57.3	58.5	1943-2059 bp	1.0	79.3
chlB-ODF51_pgt007_p4	CTGGACCAGCATACATCCAA	CAGGAGCAGGATTTGAACCT	50.0	50.0	57.3	57.5	1957-2073 bp	1.0	78.9
chlB-ODF51_pgt007_p5	CTGGACCAGCATACATCCAA	AGGATTTGAACCTGCGACTT	50.0	45.0	57.3	57.4	1950-2066 bp	1.0	78.5