Hotspot

psbD-psbA

Genome · 44470-45308 · 839 bp

Back to Desmodesmus

Pi 0.114

MarkerSeek score 87.90

Primer available yes

Label psbD-psbA

Feature Metadata

label_name: psbD-psbA
feature_type: igs
region: Genome
length_bp: 839
variable_sites: 84
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-psbA_p1	CCAGTCATCAGCGTCATCAA	AACGCTCACAACTTCCCATT	50.0	45.0	58.0	58.0	874-965 bp	1.0	91.0
psbD-psbA_p2	AACCAGTCATCAGCGTCATC	AACGCTCACAACTTCCCATT	50.0	45.0	58.0	58.0	876-967 bp	1.0	91.0
psbD-psbA_p3	CCAGTCATCAGCGTCATCAA	GCGTCTGTAGAAGCTCCTTC	50.0	55.0	58.0	58.1	847-938 bp	1.0	90.7
psbD-psbA_p4	CCAGTCATCAGCGTCATCAA	GGAAGTTATGCACGAGCGTA	50.0	50.0	58.0	58.1	893-984 bp	1.0	90.4
psbD-psbA_p5	AACCAGTCATCAGCGTCATC	GGAAGTTATGCACGAGCGTA	50.0	50.0	58.0	58.1	895-986 bp	1.0	90.4