Hotspot

ycf1

SSC · 155681-161689 · 6009 bp

Back to Desmidorchis

Pi 0.000167

MarkerSeek score 40.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6009
variable_sites: 1
pi: 0.000167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTTCGGTGGATCCCTCTT	AGTCTGGATACGGCAAAATCA	50.0	42.9	58.0	57.4	301 bp	1.0	66.9
ycf1_p2	TTCTTCGGTGGATCCCTCTT	CATGAAATGGCTTGGATTGGT	50.0	42.9	58.0	57.1	325 bp	1.0	64.7
ycf1_p3	CTTCTTCGGTGGATCCCTCT	AGTCTGGATACGGCAAAATCA	55.0	42.9	58.5	57.4	302 bp	1.0	63.2
ycf1_p4	TTCTTCGGTGGATCCCTCTT	AGTCTGGATACGGCAAAATCAT	50.0	40.9	58.0	57.8	301 bp	1.0	62.6
ycf1_p5	TTCTTCGGTGGATCCCTCTT	GTCTGGATACGGCAAAATCA	50.0	45.0	58.0	55.8	300 bp	1.0	62.6