Hotspot

psbC

LSC · 59756-61177 · 1422 bp

Back to Desmidorchis

Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TCTCAGGAAATTCGAGCAGC	AGAATGACCCGATCCCAAGA	50.0	50.0	58.0	58.1	1609 bp	1.0	79.0
psbC_p2	AAATTCGAGCAGCGGAAGAT	AGAATGACCCGATCCCAAGA	45.0	50.0	58.0	58.1	1602 bp	1.0	78.8
psbC_p3	TCTCAGGAAATTCGAGCAGC	TGACCCGATCCCAAGATGTA	50.0	50.0	58.0	57.8	1605 bp	1.0	78.6
psbC_p4	AAATTCGAGCAGCGGAAGAT	TGACCCGATCCCAAGATGTA	45.0	50.0	58.0	57.8	1598 bp	1.0	78.5
psbC_p5	AGGTATTCGTGCTTGGATGG	AGAATGACCCGATCCCAAGA	50.0	50.0	57.7	58.1	1538 bp	1.0	76.4