Hotspot

rbcL-accD

LSC · 83415-83929 · 515 bp

Back to Desmidorchis

Pi 0.00211

MarkerSeek score 62.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 515
variable_sites: 1
pi: 0.00211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	GCCATAGCGATAGACAGAGC	55.0	55.0	58.0	58.0	684-724 bp	1.0	83.7
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	AACCATTGAGTCCTCCTCCA	55.0	50.0	58.0	58.0	613-653 bp	1.0	83.5
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	GCCATAGCGATAGACAGAGC	50.0	55.0	57.9	58.0	654-694 bp	1.0	83.1
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	AACCATTGAGTCCTCCTCCA	50.0	50.0	57.9	58.0	583-623 bp	1.0	82.9
rbcL-accD_p5	AAATGGAGTCCTGAACTGGC	GCCATAGCGATAGACAGAGC	50.0	55.0	57.8	58.0	675-715 bp	1.0	82.4