Hotspot

atpB-rbcL

LSC · 81209-81986 · 778 bp

Back to Desmidorchis

Pi 0.00131

MarkerSeek score 61.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 778
variable_sites: 1
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTACATCTAGTACCGGGCCA	AACACCGGCTTTGAATCCAA	55.0	45.0	58.0	58.3	917-929 bp	1.0	78.6
atpB-rbcL_p2	GCGCAAAGGCTACATCTAGT	AACACCGGCTTTGAATCCAA	50.0	45.0	58.1	58.3	926-938 bp	1.0	78.2
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	AACACCGGCTTTGAATCCAA	55.0	45.0	58.1	58.3	856-868 bp	1.0	78.1
atpB-rbcL_p4	CGATACGTCCCAGGTTGTTT	AACACCGGCTTTGAATCCAA	50.0	45.0	57.9	58.3	887-899 bp	1.0	78.0
atpB-rbcL_p5	TCTAGTACCGGGCCAATGAT	AACACCGGCTTTGAATCCAA	50.0	45.0	57.9	58.3	912-924 bp	1.0	77.9