Hotspot

ycf1

SSC · 122655-128042 · 5388 bp

Back to Descurainia

Pi 0.0208

MarkerSeek score 49.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5388
variable_sites: 112
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTATGATCCACGGTTTGA	ATTGAGCCCTGGGTTGATAC	45.0	50.0	56.5	57.3	240 bp	1.0	65.8
ycf1_p2	TGATTGGTATGATCCACGGT	ATTGAGCCCTGGGTTGATAC	45.0	50.0	56.3	57.3	244 bp	1.0	63.9
ycf1_p3	TTGGTATGATCCACGGTTTGA	ATTGAGCCCTGGGTTGATAC	42.9	50.0	57.3	57.3	241 bp	1.0	63.8
ycf1_p4	TGGTATGATCCACGGTTTGA	GCCCTGGGTTGATACCAAG	45.0	57.9	56.5	57.8	235 bp	1.0	62.2
ycf1_p5	TGGTATGATCCACGGTTTGA	AGCCCTGGGTTGATACCAA	45.0	52.6	56.5	58.2	236 bp	1.0	62.1