Hotspot

rps15-ycf1

SSC · 122331-122654 · 324 bp

Back to Descurainia

Pi 0.0463

MarkerSeek score 68.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 324
variable_sites: 15
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGATCCCCTGCTTTC	ACACATGTATACGCGATTTCCA	50.0	40.9	57.5	58.2	417-434 bp	1.0	61.1
rps15-ycf1_p2	CAACAGATCCCCTGCTTTCT	ACACATGTATACGCGATTTCCA	50.0	40.9	57.5	58.2	416-433 bp	1.0	61.1
rps15-ycf1_p3	TCAACAGATCCCCTGCTTTC	CACATGTATACGCGATTTCCA	50.0	42.9	57.5	56.5	416-433 bp	1.0	59.0
rps15-ycf1_p4	TCAACAGATCCCCTGCTTTC	ACACATGTATACGCGATTTCC	50.0	42.9	57.5	56.5	417-434 bp	1.0	59.0
rps15-ycf1_p5	CAACAGATCCCCTGCTTTCT	CACATGTATACGCGATTTCCA	50.0	42.9	57.5	56.5	415-432 bp	1.0	58.9