Hotspot

ndhF-rpl32

SSC · 111416-112024 · 609 bp

Back to Descurainia

Pi 0.0273
MarkerSeek score 65.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
609
variable_sites
16
pi
0.0273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA ACGATGTCCAATATCCCTTCC 29.6 47.6 56.7 57.2 722-923 bp 1.0 43.8
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA ACGATGTCCAATATCCCTTCC 30.8 47.6 55.5 57.2 721-922 bp 1.0 43.8
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCCA CGATGTCCAATATCCCTTCCT 29.6 47.6 56.7 56.9 721-922 bp 1.0 43.8
ndhF-rpl32_p4 TCCATGAATATTGATATGTATGTTCCA AACGATGTCCAATATCCCTTCC 29.6 45.5 56.7 57.9 723-924 bp 1.0 43.8
ndhF-rpl32_p5 TCCATGAATATTGATATGTATGTTCCA AAGCTTTCAACGATGTCCAA 29.6 40.0 56.7 55.9 731-932 bp 1.0 43.8