Hotspot

rps11-rpl36

LSC · 78145-78263 · 119 bp

Back to Descurainia

Pi 0.0336

MarkerSeek score 65.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 4
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGAACCACTACGTGTATT	ATCCGTAGAAGGGGACGAAT	45.0	50.0	55.5	57.9	241-245 bp	1.0	61.0
rps11-rpl36_p2	CGTGAACCACTACGTGTATT	AATCCGTAGAAGGGGACGAA	45.0	50.0	55.5	58.1	242-246 bp	1.0	60.5
rps11-rpl36_p3	CGTGAACCACTACGTGTATT	TCCGTAGAAGGGGACGAATT	45.0	50.0	55.5	58.1	240-244 bp	1.0	60.5
rps11-rpl36_p4	CGTGAACCACTACGTGTATT	GTTCCAACCCGAGGCATAAA	45.0	50.0	55.5	58.2	210-214 bp	1.0	60.5
rps11-rpl36_p5	CGTGAACCACTACGTGTATT	TTCCAACCCGAGGCATAAAC	45.0	50.0	55.5	58.2	209-213 bp	1.0	60.5