Hotspot

trnD-GAC-trnY-UAC

LSC · 29778-30215 · 438 bp

Back to Descurainia

Pi 0.032

MarkerSeek score 67.60

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 14
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	509-515 bp	1.0	75.9
trnD-GAC-trnY-UAC_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	508-514 bp	1.0	75.9
trnD-GAC-trnY-UAC_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	530-536 bp	1.0	74.9
trnD-GAC-trnY-UAC_p4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.6	57.6	529-535 bp	1.0	74.7
trnD-GAC-trnY-UAC_p5	GCGGTGCTCTAACCAATTGA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.6	58.4	563-569 bp	1.0	74.3